Structural characterization of a flavin-specific RNA aptamer by chemical probing

被引：16
|
作者：
Burgstaller, P [1 ]
Famulok, M [1 ]
机构：
[1] UNIV MUNICH, GENZENTRUM, INST BIOCHEM, D-81375 MUNICH, GERMANY
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 10期
关键词：
D O I：
10.1016/0960-894X(96)00189-8
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
In a previous study, an RNA aptamer for the specific recognition of flavin cofactors was isolated from a combinatorial RNA-pool. We have now carried out a structural analysis of this RNA by chemical probing and identified residues protected from modification in the presence of the cofactor and modifications that interfere with its complexation. An induced fit of the RNA motif occurs upon cofactor binding. Our analysis led to a refined secondary structure model for the FMN/RNA complex. (C) 1996 Elsevier Science Ltd
引用
收藏
页码：1157 / 1162
页数：6
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