Using a Commercial Method for Rubisco Immunodepletion in Analysis of Plant Proteom

被引：0

作者：

Cerny, Martin ^{[1
]}

Skalak, Jan

Kurkova, Barbora

Babuliakova, Elena

Brzobohaty, Bretislav

机构：

[1] MENDELU, Lab Mol Biol Rostlin, Brno 61300, Czech Republic

来源：

CHEMICKE LISTY | 2011年 / 105卷 / 08期

关键词：

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Rubisco immunodepletion was tested using a Seppro IgY-Rubisco spin column in 2D-PAGE-based proteomic analysis. The column yield was analyzed in 20 consecutive runs and the depletion from tissues with higher and lower Rubisco contents was evaluated. An increase in the number of detected protein spots of up to 25 % was found. This procedure allows to follow the profiling of a similar number of protein spots in Rubisco-depleted proteom resolved on small gels as is usual with standard total protein extracts resolved on large gels. We found this procedure especially suitable for experiments with a limited amount of the starting material.

引用

页码：640 / 642

页数：3

共 50 条

[31] Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method
Hashiguchi, Takuyu
Hashiguchi, Masatsugu
Tanaka, Hidenori
Fukushima, Koki
Gondo, Takahiro
Akashi, Ryo
PLOS ONE, 2021, 16 (02):
[32] Analysis of Mak Diesel Engine Services at Merawang Power Plant Using FMEA Method
Gozali, Lina
Lieanda, Kenty
Jap, Lilyana
Daywin, Frans J.
TARUMANAGARA INTERNATIONAL CONFERENCE ON THE APPLICATIONS OF TECHNOLOGY AND ENGINEERING, 2019, 508
[33] Parametric optimization on exergy analysis of a thermal power plant using Taguchi design method
Baysal, Esref
Bilginsoy, Alptug Kazim
Acir, Adem
ENERGY EDUCATION SCIENCE AND TECHNOLOGY PART A-ENERGY SCIENCE AND RESEARCH, 2012, 29 (02): : 1313 - 1326
[34] Analysis of the greenness to determine risperidone in commercial products using a green stability-indicating HPTLC method
Alam, Prawez
Shakeel, Faiyaz
Alshehri, Sultan
Alhaiti, Ali
Alqarni, Mohammed H.
Foudah, Ahmed I.
Aljarba, Tariq M.
ARABIAN JOURNAL OF CHEMISTRY, 2024, 17 (04)
[35] An environmental friendly method for the automatic determination of hypochlorite in commercial products using multisyringe flow injection analysis
Ornelas Soto, N.
Horstkotte, B.
March, J. G.
Lopez de Alba, P. L.
Lopez Martinez, L.
Cerda Martin, V.
ANALYTICA CHIMICA ACTA, 2008, 611 (02) : 182 - 186
[36] Simultaneous Analysis of Seven Neonicotinoids in Commercial Milk Samples Using an UHPLC-MS/MS Method
Huang, Yu-Fang
Huang, Yi-Min
Lee, Hsin-Jui
APPLIED SCIENCES-BASEL, 2020, 10 (19):
[37] A flexible interactive coordination control method of commercial virtual power plant based on WCVAR
Hu, Shenghong
Chen, Yulin
Feng, Jian
INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS, 2024, 160
[38] Plant-wide Control of a Gas Plant Using a Structured Method
Salahshoor, Karim
Mirzae, Amin
2010 CHINESE CONTROL AND DECISION CONFERENCE, VOLS 1-5, 2010, : 1111 - 1116
[39] Optimization of a cogeneration plant using pinch method
Dragicevic, Viktor
Prelec, Zmagoslav
Frankovic, Bernard
ENERGY AND THE ENVIRONMENT 2006, VOL II, 2006, : 411 - +
[40] DETERMINATION OF CREATININE IN SERUM BY USING CREATININASE - ENHANCEMENT OF A COMMERCIAL METHOD
LANDESMAN, PW
LOTT, JA
CLINICAL CHEMISTRY, 1983, 29 (11) : 2002 - 2003

← 1 2 3 4 5 →