Identification of Enterobacteriaceae and detection of carbapenemases from positive blood cultures by combination of MALDI-TOF MS and Carba NP performed after four hour subculture in Mueller Hinton

被引:10
|
作者
Fernandez, Javier [1 ,2 ]
Rodriguez-Lucas, Carlos [2 ]
Fernandez-Suarez, Jonathan [2 ]
Vazquez, Fernando [1 ,2 ,3 ]
Rosario Rodicio, M. [1 ]
机构
[1] Univ Oviedo, Area Microbiol, Dept Biol Func, Oviedo, Spain
[2] Hosp Univ Cent Asturias, Serv Microbiol, Oviedo, Asturias, Spain
[3] Inst Univ Oftalmol Fernandez Vega, Oviedo, Asturias, Spain
关键词
MALDI-TOF MS; Carba NP; Blood cultures; Carbapenemase detection; DESORPTION-IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; RAPID IDENTIFICATION; SEPTIC SHOCK; MANAGEMENT; SPECIMENS;
D O I
10.1016/j.mimet.2016.08.014
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:133 / 135
页数:3
相关论文
共 50 条
  • [21] Direct Detection of KPC Peak from Positive Blood Cultures Using MALDI-TOF MS: Are We There Yet?
    Moreira, Natalia Kehl
    Wilhelm, Camila Morschbacher
    Echevarria, Ayme Duarte
    Volpato, Fabiana Caroline Zempulski
    Wink, Priscila Lamb
    Barth, Afonso Luis
    Caierao, Juliana
    ANTIBIOTICS-BASEL, 2023, 12 (03):
  • [22] Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method
    Yonetani, Shota
    Ohnishi, Hiroaki
    Ohkusu, Kiyofumi
    Matsumoto, Tetsuya
    Watanabe, Takashi
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2016, 52 : 37 - 42
  • [23] Impact of rapid identification by MALDI-TOF MS from positive blood cultures in Enterococcus spp. bloodstream infections
    Lopes, Diogo
    Grandbastien, Bruno
    Orasch, Christina
    Greub, Gilbert
    Croxatto, Antony
    Prod'Hom, Guy
    Guery, Benoit
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2025,
  • [24] The Clinical Impact of Rapid, Direct MALDI-ToF Identification of Bacteria from Positive Blood Cultures
    French, Kathryn
    Evans, Jason
    Tanner, Hannah
    Gossain, Savita
    Hussain, Abid
    PLOS ONE, 2016, 11 (12):
  • [25] Identification of Staphylococci Directly from Positive Blood Culture Bottles by MALDI-TOF MS System
    Kilic, Abdullah
    Baysallar, Mehmet
    MIKROBIYOLOJI BULTENI, 2014, 48 (03): : 377 - 384
  • [26] Cheap and rapid in-house method for direct identification of positive blood cultures by MALDI-TOF MS technology
    Maya Azrad
    Yoram Keness
    Orna Nitzan
    Nina Pastukh
    Linda Tkhawkho
    Victoria Freidus
    Avi Peretz
    BMC Infectious Diseases, 19
  • [27] Cheap and rapid in-house method for direct identification of positive blood cultures by MALDI-TOF MS technology
    Azrad, Maya
    Keness, Yoram
    Nitzan, Orna
    Pastukh, Nina
    Tkhawkho, Linda
    Freidus, Victoria
    Peretz, Avi
    BMC INFECTIOUS DISEASES, 2019, 19 (1)
  • [28] Evaluation of the ceftazidime/avibactam and meropenem susceptibility by MALDI-TOF MS directly from positive blood cultures
    Wilhelm, Camila Morschbacher
    Moreira, Natalia Kehl
    Inamine, Everton
    Caierao, Juliana
    Barth, Afonso Luis
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2024, 43 (01) : 171 - 175
  • [29] Evaluation of the ceftazidime/avibactam and meropenem susceptibility by MALDI-TOF MS directly from positive blood cultures
    Camila Mörschbächer Wilhelm
    Natália Kehl Moreira
    Everton Inamine
    Juliana Caierão
    Afonso Luís Barth
    European Journal of Clinical Microbiology & Infectious Diseases, 2024, 43 : 171 - 175
  • [30] Time to Positivity as a Prognostic Tool in the Performance of Short-Term Subculture for MALDI-TOF MS-Based Identification of Microorganisms from Positive Blood Cultures in Pediatric Patients
    Fang, Chao
    Zhou, Zheng
    Li, Jianping
    Zhou, Mingming
    Chen, Xuejun
    CURRENT MICROBIOLOGY, 2020, 77 (06) : 953 - 958