Identification of Enterobacteriaceae and detection of carbapenemases from positive blood cultures by combination of MALDI-TOF MS and Carba NP performed after four hour subculture in Mueller Hinton

被引:10
|
作者
Fernandez, Javier [1 ,2 ]
Rodriguez-Lucas, Carlos [2 ]
Fernandez-Suarez, Jonathan [2 ]
Vazquez, Fernando [1 ,2 ,3 ]
Rosario Rodicio, M. [1 ]
机构
[1] Univ Oviedo, Area Microbiol, Dept Biol Func, Oviedo, Spain
[2] Hosp Univ Cent Asturias, Serv Microbiol, Oviedo, Asturias, Spain
[3] Inst Univ Oftalmol Fernandez Vega, Oviedo, Asturias, Spain
关键词
MALDI-TOF MS; Carba NP; Blood cultures; Carbapenemase detection; DESORPTION-IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; RAPID IDENTIFICATION; SEPTIC SHOCK; MANAGEMENT; SPECIMENS;
D O I
10.1016/j.mimet.2016.08.014
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:133 / 135
页数:3
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