AN IMPROVED METHOD FOR EXTRACTION AND PURIFICATION OF TERMITE ENDO-β-1,4-GLUCANASE FROM FTA® CARDS

被引：6
|
作者：
Bujang, Nurmastini Sufina [1 ]
Harrison, Nigel A. [2 ]
Su, Nan-Yao [1 ]
机构：
[1] Univ Florida, Dept Entomol & Nematol, Ft Lauderdale Res & Educ Ctr, Inst Food & Agr Sci, Ft Lauderdale, FL 33314 USA
[2] Univ Florida, Dept Plant Pathol, Ft Lauderdale Res & Educ Ctr, Inst Food & Agr Sci, Ft Lauderdale, FL 33314 USA
来源：
FLORIDA ENTOMOLOGIST | 2011年 / 94卷 / 02期
关键词：
FILTER-PAPER; STORAGE; DNA; SAMPLES;
D O I：
10.1653/024.094.0236
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
In conclusion, we report an improved method for extraction and purification of termite nucleic acid from the FTA® Plantsaver Card (Whatman), which involves elution with TE buffer (pH 8) and purification with Wizard® DNA Clean-Up System (Promega). Our DNA recovery protocol requires less material, equipment, preparation, and manipulation, and reduces processing time, costs and chances for contamination compared with the manufacturer-recommended FTA® purification protocol. Most important, in relation to our study, it has been consistently effective in obtaining termite nucleic acid permitting further DNA analysis when all other methods have failed.
引用
收藏
页码：356 / 358
页数：3
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