Purification and characterization of a novel endo-β-1,4-glucanase from the thermoacidophilic Aspergillus terreus

被引：33

作者：

Gao, Jianmin ^{[1
]}

Weng, Haibo ^{[1
]}

Xi, Yu ^{[1
]}

Zhu, Daheng ^{[1
]}

Han, Shaoyin ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 02期

关键词：

Aspergillus terreus; characterization; endoglucanase; thermoacidophilic;

D O I：

10.1007/s10529-007-9536-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An endo-beta-1,4-glucanase from a thermoacidophilic fungus, Aspergillus terreus M11, was purified 18-fold with 14% yield and a specific activity of 67 U mg(-1) protein. The optimal pH was 2 and the cellulase was stable from pH 2 to 5. The cellulase had a temperature optimum of 60 degrees C measured over 30 min and retained more than 60% of its activity after heating at 70 degrees C for 1 h. The molecular mass of the cellulase was about 25 kDa. Its activity was inhibited by 77% by Hg2+ (2 mM) and by 59% by Cu2+ (2 mM).

引用

页码：323 / 327

页数：5

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