A discriminant based charge deconvolution analysis pipeline for protein profiling of whole cell extracts using liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry

被引：4
|
作者：
Lu, Weiying [1 ]
Callahan, John H. [2 ]
Fry, Frederick S. [2 ]
Andrzejewski, Denis [2 ]
Musser, Steven M. [2 ]
Harrington, Peter de B. [1 ]
机构：
[1] Ohio Univ, Dept Chem & Biochem, Clippinger Labs, Ctr Intelligent Chem Instrumentat, Athens, OH 45701 USA
[2] US FDA, Instrumentat & Biophys Branch, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA
来源：
TALANTA | 2011年 / 84卷 / 04期
关键词：
Liquid chromatography-electrospray ionization mass spectrometry; Fuzzy rule-building expert system; Molecular weight determination charge state deconvolution; Discriminant based charge deconvolution; Salmonella enterica serovar typhimurium; BUILDING EXPERT-SYSTEMS; GAS-CHROMATOGRAPHY; SPECTRA; CLASSIFICATION; VALIDATION; PROGRAM;
D O I：
10.1016/j.talanta.2011.03.024
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A discriminant based charge deconvolution analysis pipeline is proposed. The molecular weight determination (MoWeD) charge deconvolution method was applied directly to the discrimination rules obtained by the fuzzy rule-building expert system (FuRES) pattern classifier. This approach was demonstrated with synthetic electrospray ionization-mass spectra. Identification of the tentative protein biomarkers by bacterial cell extracts of Salmonella enterica serovar typhimurium strains A1 and A19 by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was also demonstrated. The data analysis time was reduced by applying this approach. In addition, this method was less affected by noise and baseline drift. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：1180 / 1187
页数：8
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