G(alpha q/11) mediates cholecystokinin activation of the cationic conductance in rat substantia nigra dopaminergic neurons

被引:0
|
作者
Wu, T
Wang, HL
机构
[1] CHANG GUNG MED COLL & TECHNOL,DEPT PHYSIOL,TAYUAN,TAIWAN
[2] CHANG GUNG MEM HOSP,DEPT NEUROL,TAYUAN,TAIWAN
关键词
sulfated cholecystokinin octapeptide; cationic currents; pertussis toxin-insensitive G proteins; whole-cell patch-clamp recordings; single-cell reverse transcriptase-polymerase chain reaction assay;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using acutely isolated rat substantia nigra neurons, our previous studies indicated that sulfated cholecystokinin octapeptide (CCK-8) excites substantia nigra dopaminergic neurons by increasing the cationic conductance and that pertussis toxin-insensitive G proteins mediate CCK-8 induction of cationic currents. G(alpha q) and G(alpha 11) are expressed in various tissues, including the brain, and likely to mediate pertussis toxin-insensitive neural signal transductions. In the present study, two different experiments were performed to test the hypothesis that G(alpha q/11) mediates CCK-8 enhancement of the cationic conductance. First, we investigated the expression of G(alpha q) and G(alpha 11) mRNAs in CCK-8-responsive substantia nigra dopaminergic neurons by combining whole-cell patch-clamp recordings with a single-cell reverse transcriptase-polymerase chain reaction assay. After CCK-8-evoked cationic currents were recorded, cellular RNA was harvested from single neurons and used as a template for the subsequent reverse transcriptase-polymerase chain reaction analysis. G(alpha q) and (G alpha 11) mRNAs were present in all substantia nigra dopaminergic neurons that responded to CCK-8. Substantia nigra dopaminergic neurons were also internally perfused with the antibody raised against the common C-terminus of G(alpha q) and (G alpha 11) during whole-cell recordings. CCK-8 failed to induce cationic currents after dopaminergic neurons were dialyzed with the anti-G(alpha q/11) antibody. Our studies suggest that CCK-8 activation of the cationic conductance in substantia nigra dopaminergic neurons is transduced by G(alpha q) and G(alpha 11).
引用
收藏
页码:1060 / 1066
页数:7
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