Production of a highly glucose-tolerant extracellular β-glucosidase by three Aspergillus strains

被引：49

作者：

Günata, Z ^{[1
]}

Vallier, MJ ^{[1
]}

机构：

[1] INRA, Lab Aromes & Subst Nat, IPV, F-34060 Montpellier, France

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 03期

关键词：

Aspergillus; glucose-tolerant beta-glucosidase; quercetin; production;

D O I：

10.1023/A:1005407710806

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Quercetin was the best inducer for the production of a highly glucose-tolerant, extracellular beta-glucosidase in Aspergillus niger and Aspergillus oryzae. The enzyme was separated from the major and common beta-glucosidase by gel filtration and that from Aspergillus oryzae further purified by ion-exchange chromatography. It was highly resistant to glucose inhibition (K-i = 953 mM), had a pI of 4.2, optimum pH of 4.5-6.0 and a molecular mass of 30 kDa according to gel filtration. The enzyme was active against cellobiose and alkyl glucosides.

引用

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页码：219 / 223

页数：5

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