NCAPH Is Required for Proliferation, Migration and Invasion of Non-small-cell Lung Cancer Cells

被引：23
|
作者：
Kim, Byeol [1 ]
Kim, Seok Won [2 ]
Lim, Ji-Yeon [1 ]
Park, Seon-Joo [1 ]
机构：
[1] Chosun Univ, Coll Med, Dept Premed Sci, 309 Philmoon Daero, Gwangju 61452, South Korea
[2] Chosun Univ, Coll Med, Dept Neurosurg, Gwangju, South Korea
来源：
ANTICANCER RESEARCH | 2020年 / 40卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
NCAPH; condensin; chromosome; cell cycle; NSCLC; CONDENSIN-I; TARGETS;
D O I：
10.21873/anticanres.14305
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) is implicated in correct chromosome condensation and segregation during mitosis. However, the functional role of NCAPH in the pathogenesis of non-small-cell lung cancer (NSCLC) remains unclear. The aim of this study was to elucidate the role of NCAPH in NSCLC cells. Materials and Methods: A549 and H1299 NSCLC cells were transfected with small-interfering RNA (siRNA) against NCAPH. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony-formation assay and flow cytometry analysis were performed to reveal the role of NCAPH in NSCLC cells. In addition, migration and invasion assay were also performed. Results: NCAPH knockdown inhibited cell proliferation, induced cell-cycle arrest at G(2)/M phase, and prevented colony formation, migration and invasion by NSCLC cells. Conclusion: NCAPH is involved in NSCLC progression and development, and may be a potential therapeutic target for NSCLC treatment.
引用
收藏
页码：3239 / 3246
页数：8
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