Immobilization of protein molecules on step-controlled sapphire surfaces

被引：15

作者：

Aoki, R.

Arakawa, T.

Misawa, N.

Tero, R.

Urisu, T.

Takeuchi, A.

Ogino, T. ^{[1
]}

机构：

[1] Yokohama Natl Univ, Yokohama, Kanagawa 240, Japan

[2] Univ Tokyo, Tokyo, Japan

来源：

SURFACE SCIENCE | 2007年 / 601卷 / 21期

基金：

日本科学技术振兴机构;

关键词：

atomic force microscopy; adhesion; biological molecules-proteins; aluminum oxide; steps;

D O I：

10.1016/j.susc.2007.08.018

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have studied effects of surface morphology on immobilization of protein molecules using step-controlled sapphire surfaces. Preferential adsorption of avidin molecules on the step edges was observed on the single-stepped sapphire surface. A randomly-stepped sapphire surface was found to be suitable for high-density immobilization of protein molecules. These results indicate atomic scale structures of the substrate surface influence the adsorption efficiency of the proteins. By using an atomic force microscopy (AFM) equipped with a biotin-modified cantilever, we have confirmed that the immobilized avidin molecules on the substrates keep their biological activity. This means that the ligand-receptor interaction can be detected using the phase image mode of a standard AFM. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：4915 / 4921

页数：7

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