Emerging Separation Techniques in Supercritical Fluid Chromatography

被引：5

作者：

Yamamoto, Kazuhiro ^{[1
]}

Machida, Koichi ^{[1
]}

Kotani, Akira ^{[1
]}

Hakamata, Hideki ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2021年 / 69卷 / 10期

关键词：

supercritical fluid chromatography; separation technique; biological molecule; GC X GC; BIOACTIVE REDOX COMPONENTS; GAS-CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; ELECTROCHEMICAL DETECTION; AMINO-ACIDS; SPECTROMETRY; PURIFICATION; COLUMNS; WATER;

D O I：

10.1248/cpb.c21-00306

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Supercritical fluid chromatography (SFC) has unique separative characteristics distinguished from those of HPLC and gas chromatography. At present, SFC is widely used and there are many applications in various biological, medical, and pharmaceutical fields. In this review, we focus on recently developed novel techniques related to SFC separation including: new column stationary phases, microfluidics, two-dimensional separation, and gas-liquid separation. In addition, we discuss the application of SFC using a water-containing modifier to biological molecules such as amino acids, peptides, and small proteins that had been challenging analytes.

引用

页码：970 / 975

页数：6

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