Chronopotentiometric Technique as a Method for Electrical Characterization of Bilayer Lipid Membranes

被引:18
|
作者
Naumowicz, Monika [1 ]
Figaszewski, Zbigniew Artur [1 ,2 ]
机构
[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
[2] Univ Warsaw, Fac Chem, Lab Electrochem Power Sources, PL-02093 Warsaw, Poland
来源
JOURNAL OF MEMBRANE BIOLOGY | 2011年 / 240卷 / 01期
关键词
Chronopotentiometry; Bilayer lipid membrane; Phosphatidylcholine; Capacitance; Resistance; OXIDIZED-CHOLESTEROL; ELECTROPORATION; CAPACITANCE; SYSTEM; CONDUCTANCE; POTENTIALS; CHARGE; PORES;
D O I
10.1007/s00232-011-9341-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The basic electrical parameters of bilayer lipid membranes are capacitance and resistance. This article describes the application of chronopotentiometry to the research of lipid bilayers. Membranes were made from egg yolk phosphatidylcholine. The chronopotentiometric characteristic of the membranes depends on the current value. For low current values, no electroporation takes place and the voltage rises exponentially to a constant value. Based on these kinds of chronopotentiometric curves, a method of the membrane capacitance and the membrane resistance calculations are presented.
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页码:47 / 53
页数:7
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