Chronopotentiometric Technique as a Method for Electrical Characterization of Bilayer Lipid Membranes

被引:18
|
作者
Naumowicz, Monika [1 ]
Figaszewski, Zbigniew Artur [1 ,2 ]
机构
[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
[2] Univ Warsaw, Fac Chem, Lab Electrochem Power Sources, PL-02093 Warsaw, Poland
来源
JOURNAL OF MEMBRANE BIOLOGY | 2011年 / 240卷 / 01期
关键词
Chronopotentiometry; Bilayer lipid membrane; Phosphatidylcholine; Capacitance; Resistance; OXIDIZED-CHOLESTEROL; ELECTROPORATION; CAPACITANCE; SYSTEM; CONDUCTANCE; POTENTIALS; CHARGE; PORES;
D O I
10.1007/s00232-011-9341-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The basic electrical parameters of bilayer lipid membranes are capacitance and resistance. This article describes the application of chronopotentiometry to the research of lipid bilayers. Membranes were made from egg yolk phosphatidylcholine. The chronopotentiometric characteristic of the membranes depends on the current value. For low current values, no electroporation takes place and the voltage rises exponentially to a constant value. Based on these kinds of chronopotentiometric curves, a method of the membrane capacitance and the membrane resistance calculations are presented.
引用
收藏
页码:47 / 53
页数:7
相关论文
共 50 条
  • [1] Chronopotentiometric Technique as a Method for Electrical Characterization of Bilayer Lipid Membranes
    Monika Naumowicz
    Zbigniew Artur Figaszewski
    [J]. The Journal of Membrane Biology, 2011, 240 : 47 - 53
  • [2] Chronoamperometric Technique as a Useful Method for Electrical Characterization of Bilayer Lipid Membranes
    Naumowicz, Monika
    Figaszewski, Zbigniew Artur
    [J]. JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2014, 161 (01) : H11 - H16
  • [3] Chronopotentiometric studies of electroporation of bilayer lipid membranes
    Kalinowski, S
    Ibron, G
    Bryl, K
    Figaszewski, Z
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1369 (02): : 204 - 212
  • [4] Influence of cholesterol on electroporation of bilayer lipid membranes: chronopotentiometric studies
    Koronkiewicz, S
    Kalinowski, S
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2004, 1661 (02): : 196 - 203
  • [5] Electrical properties of supported lipid bilayer membranes
    Wiegand, G
    Arribas-Layton, N
    Hillebrandt, H
    Sackmann, E
    Wagner, P
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (16): : 4245 - 4254
  • [6] SOME ELECTRICAL PROPERTIES OF LIPID BILAYER MEMBRANES
    MIYAMOTO, VK
    THOMPSON, TE
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1967, 25 (01) : 16 - &
  • [7] ELECTRICAL BREAKDOWN OF BILAYER LIPID-MEMBRANES
    CHIZMADZHEV, Y
    PASTUSHENKO, V
    [J]. STUDIA BIOPHYSICA, 1978, 74 : 29 - 30
  • [8] ELECTRICAL RESPONSES TO VIBRATION IN LIPID BILAYER MEMBRANES
    OCHS, AL
    BURTON, RM
    [J]. BIOPHYSICAL JOURNAL, 1968, 8 : A27 - &
  • [9] ELECTRICAL BREAKDOWN OF BILAYER LIPID-MEMBRANES
    ABIDOR, IG
    ARAKELIAN, VB
    PASTUSHENKO, VF
    TARASEVICH, MR
    CHERNOMORDIK, LV
    CHIZMADZHEV, IA
    [J]. DOKLADY AKADEMII NAUK SSSR, 1978, 240 (03): : 733 - 736
  • [10] Electrical properties of planar bilayer lipid membranes
    Zhang, YF
    Hou, XP
    [J]. MOLECULAR CRYSTALS AND LIQUID CRYSTALS SCIENCE AND TECHNOLOGY SECTION A-MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 1999, 337 : 469 - 472