Agromyces flavus sp nov., an actinomycete isolated from soil

A Gram-positive, non-motile strain, designated CPCC 202695(T), was isolated from a soil sample collected from the Qinghai-Tibet plateau, north-west China. Strain CPCC 202695T contained rhamnose, glucose and galactose in the cell wall as diagnostic sugars and 2,4-diaminobutyric acid, alanine, glutamic acid and glycine in the peptidoglycan. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. MK-12 was the predominant menaquinone and anteiso-C(15:0) (34.2%), iso-C(15:0) (19.8%), iso-C(16:0) (12.7%) and anteiso-C(17:0) (11.1%) were the major fatty acids. 16S rRNA gene sequence similarities (94.2-97.1%) between the isolate and the type strains of recognized species of the genus Agromyces indicated that strain CPCC 202695(T) was a member of the genus Agromyces. DNA DNA relatedness clearly separated strain CPCC 202695(T) from its closest relatives. The phenotypic and genotypic data demonstrated that strain CPCC 202695(T) represents a novel species of the genus Agromyces, for which the name Agromyces flavus sp. nov. is proposed. The type strain is CPCC 202695(T) (=KCTC 19578(T) =CCM 7623(T)).