Orthogonal protocols for DNA extraction from filamentous fungi

被引：19
|
作者：
Conlon, Benjamin H. [1 ]
Schmidt, Suzanne [1 ]
Poulsen, Michael [1 ]
Shik, Jonathan Z. [1 ,2 ]
机构：
[1] Univ Copenhagen, Dept Biol, Sect Ecol & Evolut, Copenhagen, Denmark
[2] Smithsonian Trop Res Inst, Apartado Postal 084303092, Balboa, Ancon, Panama
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 01期
基金：
欧洲研究理事会;
关键词：
Genetics; Genomics; Microbiology; Molecular Biology;
D O I：
10.1016/j.xpro.2022.101126
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).
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收藏
页数：11
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