Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

To study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor beta (TGF-beta)/Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor alpha (TNF-alpha), and the anti-adhesion mechanism of LNNS in the abdominal cavity. The primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identifified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-alpha; RPMC treated by a low-dosage LNNS group (2.5 mg/L); RPMC treated by a medium-dosage LNNS group (5 mg/L); and RPMC treated by a high-dosage LNNS group (10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fifibronectin, collagen I (COL-I), TGF-beta mRNA, and Western blot method to test the Smad protein 7 expression of RPMC. Compared with the blank group, a signifificant elevation in fifibronectin (FN), COL-I and TGF-beta mRNA expression of RPMC were observed in the control group (P < 0.05). Compared with the control group, LNNS suppressed the expressions of FN, COL-I and TGF-beta mRNA in a concentrationdependent manner (P < 0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-alpha stimulation, and up-regulated with the increase of LNNS dose (P < 0.05). TNF-alpha may induce changes in RPMC's viability, leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN, COL-I and TGF-beta, up-regulating the expression of Smad7 by TNF-alpha in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.