Purification and characterization of pectin methylesterase from acerola (Malpighia glabra L.)

被引：15
|
作者：
de Assis, Sandra Aparecida
Martins, Antönio Baldo Geraldo
de Faria Oliveira, Olga Maria Mascarenhas
机构：
[1] UNESP, Dept Bioquim & Tecnol Quim, Inst Quim, BR-14801970 Araraquara, SP, Brazil
[2] Univ Estadual Feira de Santana, Dept Saude, BR-44031460 Feira De Santana, BA, Brazil
[3] UNESP, Dept Hort, Jaboticabal, SP, Brazil
来源：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2007年 / 87卷 / 10期
关键词：
pectinmethylesterase; acerola; kinetic characterization; purification; isoenzymes; heat stability;
D O I：
10.1002/jsfa.2884
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The enzyme pectinmethylesterase (PME) from acerola was extracted and purified by gel anion-exchange chromatography (Q Sepharose) and filtration on Sephadex G-100. The results showed two different PME isoforms (PME1 and PME2), with molecular masses of 25.10 and 5.20 kDa, respectively. PMEI specific activity increased by 9.63% after 60 min incubation at 98 degrees C, while PME2 retained 66% of its specific activity under the same conditions. The K-m values of PMEI, PME2 and concentrated PME were 0.94, 0.08 and 0.08mg mL(-1), respectively. The V-max value of PMEI, PME2 and concentrated were 204.08, 2, 158.73 and 2.92 mu mol min(-1) mg(-1) protein, respectively. (c) 2007 Society of Chemical Industry.
引用
收藏
页码：1845 / 1849
页数：5
相关论文
共 50 条
  • [21] Sensorial Analysis of Wines from Malpighia glabra L. Pulp
    Almeida, Sheyla dos Santos
    de Souza, Roberto Rodrigues
    Curvelo Santana, Jose Carlos
    Tambourgi, Elias Basile
    [J]. BRAZILIAN JOURNAL OF OPERATIONS & PRODUCTION MANAGEMENT, 2008, 5 (01): : 63 - 72
  • [22] Partial purification and characterization of pectin methylesterase from green beans (Phaseolus vulgaris L)
    Laats, MM
    Grosdenis, F
    Recourt, K
    Voragen, AGJ
    Wichers, HJ
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (03) : 572 - 577
  • [23] Leaf anatomy of Malpighia glabra L. (Malpighiaceae)
    Soares, MKM
    Servin, LFV
    Appezzato-da-Gloria, B
    [J]. ARQUIVOS DE BIOLOGIA E TECNOLOGIA, 1997, 40 (03): : 738 - 746
  • [24] Volatile constituents of fruits of Malpighia glabra L.
    Casadebaig, J
    Pélissier, Y
    Marion, C
    Milhau, M
    Bessière, JM
    [J]. JOURNAL OF ESSENTIAL OIL RESEARCH, 2000, 12 (06) : 677 - 678
  • [25] Rooting on acerola (Malpighia glabra L. and M-emarginata Sesse & Moc. ex DC) stakes
    Moratinos, P.
    Flores, E.
    Gomez, A.
    Ramirez-Villalobos, M.
    [J]. REVISTA DE LA FACULTAD DE AGRONOMIA DE LA UNIVERSIDAD DEL ZULIA, 2008, 25 (03): : 405 - 420
  • [26] Osmotic dehydration of acerola fruit (Malpighia punicifolia L.)
    Alves, DG
    Barbosa, JL
    Antonio, GC
    Murr, FEX
    [J]. JOURNAL OF FOOD ENGINEERING, 2005, 68 (01) : 99 - 103
  • [27] Isolation, purification, and characterization of pectin methylesterase inhibitor and polygalacturonase inhibitor protein from Indian lemon (Citrus limon L.)
    Bakshi, Gayatri
    Ananthanarayan, Laxmi
    [J]. PHYTOCHEMISTRY, 2021, 189
  • [28] Partial purification, characterization, and thermal and high-pressure inactivation of pectin methylesterase from carrots (Daucus carrota L.)
    Ly-Nguyen, B
    Van Loey, AM
    Fachin, D
    Verlent, I
    Indrawati
    Hendrickx, ME
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (19) : 5437 - 5444
  • [29] Cytotoxic and mutagenic effects of iodine-131 and radioprotection of acerola (Malpighia glabra L.) and beta-carotene in vitro
    Almeida, I. V.
    Duesman, E.
    Heck, M. C.
    Pamphile, J. A.
    Lopes, N. B.
    Tonin, L. T. D.
    Vicentini, V. E. P.
    [J]. GENETICS AND MOLECULAR RESEARCH, 2013, 12 (04): : 6402 - 6413
  • [30] INFLUENCE OF MATURITY STAGES AND STORAGE-CONDITIONS ON THE CONSERVATION OF ACEROLA (MALPIGHIA-GLABRA L)
    DECARVALHO, RIN
    MANICA, I
    [J]. PESQUISA AGROPECUARIA BRASILEIRA, 1994, 29 (05) : 681 - 688
12345