Comparative Pathogenicity and Transmissibility of Pandemic H1N1, Avian H5N1, and Human H7N9 Influenza Viruses in Tree Shrews

被引:13
|
作者
Xu, Shuai [1 ]
Li, Xuyong [1 ]
Yang, Jiayun [1 ]
Wang, Zhengxiang [1 ]
Jia, Yane [1 ]
Han, Lu [1 ]
Wang, Liang [1 ]
Zhu, Qiyun [1 ]
机构
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China
来源
FRONTIERS IN MICROBIOLOGY | 2019年 / 10卷
基金
中国国家自然科学基金;
关键词
tree shrew; infectivity; transmissibility; HUMAN INFECTION; ANIMAL-MODELS; A VIRUSES; SWINE; REPLICATION; ADAPTATION; PROTECTION; FERRETS; CHINA;
D O I
10.3389/fmicb.2019.02955
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Influenza A viruses (IAVs) continuously challenge the poultry industry and human health. Studies of IAVs are still hampered by the availability of suitable animal models. Chinese tree shrews (Tupaia belangeri chinensis) are closely related to primates physiologically and genetically, which make them a potential animal model for human diseases. In this study, we comprehensively evaluated infectivity and transmissibility in Chinese tree shrews by using pandemic H1N1 (A/Sichuan/1/2009, pdmH1N1), avian-origin H5N1 (A/Chicken/Gansu/2/2012, H5N1) and early human-origin H7N9 (A/Suzhou/SZ19/2014, H7N9) IAVs. We found that these viruses replicated efficiently in primary tree shrew cells and tree shrews without prior adaption. Pathological lesions in the lungs of the infected tree shrews were severe on day 3 post-inoculation, although clinic symptoms were self-limiting. The pdmH1N1 and H7N9 viruses, but not the H5N1 virus, transmitted among tree shrews by direct contact. Interestingly, we also observed that unadapted H7N9 virus could transmit from tree shrews to naive guinea pigs. Virus-inoculated tree shrews generated a strong humoral immune response and were protected from challenge with homologous virus. Taken together, our findings suggest the Chinese tree shrew would be a useful mammalian model to study the pathogenesis and transmission of IAVs.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Update on avian influenza A(H5N1) and A(H7N9)
    Kaye, Donald
    [J]. CLINICAL INFECTIOUS DISEASES, 2017, 64 (07) : I - I
  • [2] Comparison of Patients Hospitalized With Influenza A Subtypes H7N9, H5N1, and 2009 Pandemic H1N1
    Wang, Chen
    Yu, Hongjie
    Horby, Peter W.
    Cao, Bin
    Wu, Peng
    Yang, Shigui
    Gao, Hainv
    Li, Hui
    Tsang, Tim K.
    Liao, Qiaohong
    Gao, Zhancheng
    Ip, Dennis K. M.
    Jia, Hongyu
    Jiang, Hui
    Liu, Bo
    Ni, Michael Y.
    Dai, Xiahong
    Liu, Fengfeng
    Nguyen Van Kinh
    Nguyen Thanh Liem
    Tran Tinh Hien
    Li, Yu
    Yang, Juan
    Wu, Joseph T.
    Zheng, Yaming
    Leung, Gabriel M.
    Farrar, Jeremy J.
    Cowling, Benjamin J.
    Uyeki, Timothy M.
    Li, Lanjuan
    [J]. CLINICAL INFECTIOUS DISEASES, 2014, 58 (08) : 1095 - 1103
  • [3] Differences in the Epidemiology of Human Cases of Avian Influenza A(H7N9) and A(H5N1) Viruses Infection
    Qin, Ying
    Horby, Peter W.
    Tsang, Tim K.
    Chen, Enfu
    Gao, Lidong
    Ou, Jianming
    Tran Hien Nguyen
    Tran Nhu Duong
    Gasimov, Viktor
    Feng, Luzhao
    Wu, Peng
    Jiang, Hui
    Ren, Xiang
    Peng, Zhibin
    Li, Sa
    Li, Ming
    Zheng, Jiandong
    Liu, Shelan
    Hu, Shixiong
    Hong, Rongtao
    Farrar, Jeremy J.
    Leung, Gabriel M.
    Gao, George F.
    Cowling, Benjamin J.
    Yu, Hongjie
    [J]. CLINICAL INFECTIOUS DISEASES, 2015, 61 (04) : 563 - 571
  • [4] Differences in the Epidemiology of Childhood Infections with Avian Influenza A H7N9 and H5N1 Viruses
    Sha, Jianping
    Dong, Wei
    Liu, Shelan
    Chen, Xiaowen
    Zhao, Na
    Luo, Mengyun
    Dong, Yuanyuan
    Zhang, Zhiruo
    [J]. PLOS ONE, 2016, 11 (10):
  • [5] Co-infection with Avian (H7N9) and Pandemic (H1N1) 2009 Influenza Viruses, China
    Zhang, Wanju
    Zhu, Dongyi
    Tian, Di
    Xu, Lei
    Zhu, Zhaokui
    Teng, Zheng
    He, Jing
    Shan, Shan
    Liu, Yi
    Wang, Wei
    Yuan, Zhenghong
    Ren, Tao
    Hu, Yunwen
    [J]. EMERGING INFECTIOUS DISEASES, 2015, 21 (04) : 715 - 718
  • [6] Comparative Epidemiology of Human Fatal Infections with Novel, High (H5N6 and H5N1) and Low (H7N9 and H9N2) Pathogenicity Avian Influenza A Viruses
    Wu, Zu-Qun
    Zhang, Yi
    Zhao, Na
    Yu, Zhao
    Pan, Hao
    Chan, Ta-Chien
    Zhang, Zhi-Ruo
    Liu, She-Lan
    [J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2017, 14 (03):
  • [7] Biosensing methods for the detection of highly pathogenic avian influenza H5N1 and H7N9 viruses
    Li, Zhanming
    Fu, Yingchun
    Liao, Ming
    Li, Yanbin
    [J]. ANALYTICAL METHODS, 2017, 9 (36) : 5238 - 5248
  • [8] H7N9死亡风险低于H5N1高于H1N1
    王莉
    [J]. 海南医学, 2013, 24 (15) : 2323 - 2323
  • [9] Identification of climate factors related to human infection with avian influenza A H7N9 and H5N1 viruses in China
    Jing Li
    Yuhan Rao
    Qinglan Sun
    Xiaoxu Wu
    Jiao Jin
    Yuhai Bi
    Jin Chen
    Fumin Lei
    Qiyong Liu
    Ziyuan Duan
    Juncai Ma
    George F. Gao
    Di Liu
    Wenjun Liu
    [J]. Scientific Reports, 5
  • [10] Identification of climate factors related to human infection with avian influenza A H7N9 and H5N1 viruses in China
    Li, Jing
    Rao, Yuhan
    Sun, Qinglan
    Wu, Xiaoxu
    Jin, Jiao
    Bi, Yuhai
    Chen, Jin
    Lei, Fumin
    Liu, Qiyong
    Duan, Ziyuan
    Ma, Juncai
    Gao, George F.
    Liu, Di
    Liu, Wenjun
    [J]. SCIENTIFIC REPORTS, 2015, 5