A "Turn-On" Fluorescence Copper Biosensor Based on DNA Cleavage-Dependent Graphene Oxide-dsDNA-CdTe Quantum Dots Complex

被引：7

作者：

Ding, Liyun ^{[1
]}

Xu, Bing ^{[1
]}

Li, Tao ^{[1
]}

Huang, Jun ^{[1
]}

Bai, Wei ^{[2
]}

机构：

[1] Wuhan Univ Technol, Natl Engn Lab Fiber Opt Sensing Technol, Wuhan 430070, Hubei, Peoples R China

[2] Hubei Univ Chinese Med, Sch Informat Engn, Wuhan 430065, Hubei, Peoples R China

来源：

SENSORS | 2018年 / 18卷 / 08期

基金：

中国国家自然科学基金;

关键词：

copper ions detection; dsDNA-graphene oxide; CdTe quantum dots; fluorescence recovery; COLORIMETRIC DETECTION; SENSOR; CU2+; DNAZYMES; IONS; NANOPARTICLES; NANOCOMPOSITES; SENSITIVITY; SELECTIVITY; INHIBITION;

D O I：

10.3390/s18082605

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel "turn-on" fluorescent copper biosensor is developed successfully based on the graphene oxide (GO)-dsDNA-CdTe quantum dots (QDs) complex via chemical crosslink method. The optical and structure properties of GO-dsDNA-CdTe QDs complex are studied by fluorescence (FL) spectra and transmission electron microscopy (TEM) in detail. It is demonstrated that the fluorescence quenching of CdTe QDs is a process of fluorescence resonance energy transfer (FRET) due to the essential surface and quenching properties of two-dimensional GO. Copper ions induce the catalytic reaction of DNA chain and irreversibly break at the cleavage site, which will cause the G-quadruplex formation, moreover further result in the CdTe QDs separated from GO and restored its fluorescence. Therefore, a significant recovery effect on the fluorescence of the GO-dsDNA-CdTe QDs complex is observed in the presence of copper ions. The fluorescence responses are concentration-dependent and can be well described by a linear equation. Compared with other metal ions, the sensor performs good selectivity for copper ions.

引用

页数：10

共 38 条

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