A "Turn-On" Fluorescence Copper Biosensor Based on DNA Cleavage-Dependent Graphene Oxide-dsDNA-CdTe Quantum Dots Complex

被引：7
|
作者：
Ding, Liyun [1 ]
Xu, Bing [1 ]
Li, Tao [1 ]
Huang, Jun [1 ]
Bai, Wei [2 ]
机构：
[1] Wuhan Univ Technol, Natl Engn Lab Fiber Opt Sensing Technol, Wuhan 430070, Hubei, Peoples R China
[2] Hubei Univ Chinese Med, Sch Informat Engn, Wuhan 430065, Hubei, Peoples R China
来源：
SENSORS | 2018年 / 18卷 / 08期
基金：
中国国家自然科学基金;
关键词：
copper ions detection; dsDNA-graphene oxide; CdTe quantum dots; fluorescence recovery; COLORIMETRIC DETECTION; SENSOR; CU2+; DNAZYMES; IONS; NANOPARTICLES; NANOCOMPOSITES; SENSITIVITY; SELECTIVITY; INHIBITION;
D O I：
10.3390/s18082605
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel "turn-on" fluorescent copper biosensor is developed successfully based on the graphene oxide (GO)-dsDNA-CdTe quantum dots (QDs) complex via chemical crosslink method. The optical and structure properties of GO-dsDNA-CdTe QDs complex are studied by fluorescence (FL) spectra and transmission electron microscopy (TEM) in detail. It is demonstrated that the fluorescence quenching of CdTe QDs is a process of fluorescence resonance energy transfer (FRET) due to the essential surface and quenching properties of two-dimensional GO. Copper ions induce the catalytic reaction of DNA chain and irreversibly break at the cleavage site, which will cause the G-quadruplex formation, moreover further result in the CdTe QDs separated from GO and restored its fluorescence. Therefore, a significant recovery effect on the fluorescence of the GO-dsDNA-CdTe QDs complex is observed in the presence of copper ions. The fluorescence responses are concentration-dependent and can be well described by a linear equation. Compared with other metal ions, the sensor performs good selectivity for copper ions.
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页数：10
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