Serological markers for Leishmania donovani infection in Nepal: agreement between direct agglutination test and rK39 ELISA

被引：16
|
作者：
Khanal, Basudha [2 ]
Rijal, Suman [2 ]
Ostyn, Bart
Picado, Albert [3 ]
Gidwani, Kamlesh [4 ]
Menten, Joris
Jacquet, Diane
Lejon, Veerle
Chappuis, Francois [5 ]
Boelaert, Marleen [1 ]
机构：
[1] Inst Trop Med, Dept Publ Hlth, B-2000 Antwerp, Belgium
[2] BP Koirala Inst Hlth Sci, Dharan, Nepal
[3] London Sch Hyg & Trop Med, London WC1, England
[4] Banaras Hindu Univ, Varanasi 221005, Uttar Pradesh, India
[5] Univ Hosp Geneva, Geneva, Switzerland
来源：
TROPICAL MEDICINE & INTERNATIONAL HEALTH | 2010年 / 15卷 / 11期
关键词：
visceral leishmaniasis; kala-azar; Leishmania donovani; infection markers; LINKED-IMMUNOSORBENT-ASSAY; INDIAN VISCERAL LEISHMANIASIS; RECOMBINANT K-39 ANTIGEN; KALA-AZAR; DIAGNOSIS; ANTIBODY; EPIDEMIOLOGY; DISEASE;
D O I：
10.1111/j.1365-3156.2010.02631.x
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Visceral leishmaniasis (VL) is an important vector-borne disease caused by Leishmania donovani in the Indian subcontinent. The actual incidence and role of asymptomatic infections in the region are not well known. We used the direct agglutination test (DAT) and the rK39 ELISA as L. donovani infection markers in 10 VL endemic villages in Nepal. DAT titre distribution showed two subgroups in the population (infected and non-infected individuals), while rK39 did not. The agreement between both tests was moderate (kappa = 0.53; 95% CI 0.49-0.57). More research is needed to develop validated markers for Leishmania infection.
引用
收藏
页码：1390 / 1394
页数：5
相关论文
共 25 条
  • [11] Serological studies on rK39 negative Visceral Leishmaniasis in an endemic focus of Leishmania donovani induced Cutaneous Leishmaniasis
    Deepachandi, Bhagya
    Weerasinghe, Sudath
    Soysa, Preethi
    Chatterjee, Mitali
    Siriwardana, Yamuna
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2023, 105 (03)
  • [12] Serological diagnosis of Indian visceral leishmaniasis - Direct agglutination test versus RK39 strip test and comparison of two strip formats
    Sundar, Shyam
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2005, 73 (06): : 100 - 100
  • [13] Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain
    Bangert, Mathieu
    Flores-Chavez, Maria D.
    Llanes-Acevedo, Ivonne P.
    Arcones, Carolina
    Chicharro, Carmen
    Garcia, Emilia
    Ortega, Sheila
    Nieto, Javier
    Cruz, Israel
    PLOS NEGLECTED TROPICAL DISEASES, 2018, 12 (03):
  • [14] Evaluation of the direct agglutination test and the rK39 dipstick test for the sero-diagnosis of visceral leishmaniasis
    Schallig, HDFH
    Canto-Cavalheiro, M
    da Silva, ES
    MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (07): : 1015 - 1018
  • [15] Assessment of the direct agglutination test, fast agglutination screening test, and rK39 dipstick test for the sero-diagnosis of visceral leishmaniasis in Syria
    Al-Nahhas, Samar A.
    Al-Taweel, Atef A.
    Al-Taweel, Merfat A.
    SAUDI MEDICAL JOURNAL, 2008, 29 (09) : 1250 - 1254
  • [16] A meta-analysis of the diagnostic performance of the direct agglutination test and rK39 dipstick for visceral leishmaniasis
    Chappuis, Francois
    Rijal, Suman
    Soto, Alonso
    Menten, Joris
    Boelaert, Marleen
    BMJ-BRITISH MEDICAL JOURNAL, 2006, 333 (7571): : 723 - 726
  • [17] Comparison of an rK39 dipstick rapid test with direct agglutination test and splenic aspiration for the diagnosis of kala-azar in Sudan
    Veeken, H
    Ritmeijer, K
    Seaman, J
    Davidson, R
    TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2003, 8 (02) : 164 - 167
  • [18] Multi-centric prospective evaluation of rk39 rapid test and direct agglutination test for the diagnosis of visceral leishmaniasis in Brazil
    de Assis, Talia S. M.
    Braga, Alexandre S. da C.
    Pedras, Mariana J.
    Oliveira, Edward
    Barral, Aldina
    de Siqueira, Isadora C.
    Costa, Carlos H. N.
    Costa, Dorcas L.
    Holanda, Thiago A.
    Soares, Vitor Y. R.
    Bia, Mauro
    Caldas, Arlene de J. M.
    Romero, Gustavo A. S.
    Rabello, Ana
    TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2011, 105 (02) : 81 - 85
  • [19] Diagnostic accuracy of direct agglutination test, rK39 ELISA and six rapid diagnostic tests among visceral leishmaniasis patients with and without HIV coinfection in Ethiopia
    Kassa, Mekibib
    Abdellati, Said
    Cnops, Lieselotte
    Hinckel, Bruno C. Bremer
    Yeshanew, Arega
    Hailemichael, Wasihun
    Vogt, Florian
    Adriaensen, Wim
    Mertens, Pascal
    Diro, Ermias
    van Griensven, Johan
    Van den Bossche, Dorien
    PLOS NEGLECTED TROPICAL DISEASES, 2020, 14 (12): : 1 - 13
  • [20] Field Evaluation of rK39 Test and Direct Agglutination Test for Diagnosis of Visceral Leishmaniasis in a Population with High Prevalence of Human Immunodeficiency Virus in Ethiopia
    ter Horst, Rachel
    Tefera, Tewodros
    Assefa, Gessesse
    Ebrahim, Abdurazik Z.
    Davidson, Robert N.
    Ritmeijer, Koert
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2009, 80 (06): : 929 - 934
123