The possibility of using mesenchymal stem cells for veterinary research and medicine - Part 2 Own examinations

In the first part of the two-part article series the authors introduced the possibility of the application of mesenchymal stem cell (MSC) in the field of human and principally veterinary medicine. In this second article they present their own results of the in vitro prolifer active capacity and osteogenic differentation potential of adipose tissue MSCs isolated from dogs of different ages. MSC cells were isolated from suboutaneous adipose tissue which were prodiffferated, characterized according to the international standard, differentiated and following experiments. Their observation revealed that MSCs from old and young dono. Beagle dogs prodiferated property in vitro and they were able to show the required fibrobast. like morphology and differentiated into adipo. onondro- and osteogenic lineager. The osteogenic differentiationaof MSCs was followed by the measurement of alkaline phosphetase activity and caicuum accumutaion. Both of the determined paramenters were higher in the case of younger donors ompated to the old doner dog.