The production of alkaline protease by a Bacillus species isolate

被引:65
|
作者
Mehrotra, S [1 ]
Pandey, PK [1 ]
Gaur, R [1 ]
Darmwal, NS [1 ]
机构
[1] DR RML Avadh Univ, Dept Microbiol, Faizabad, Uttar Pradesh, India
关键词
alkaline protease; Bacillus sp; alkalophiles;
D O I
10.1016/S0960-8524(98)00107-2
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Alkalophilic bacterial strains (52) isolated from saline-alkali soils were screened on milk agar medium for their ability to produce alkaline protease. Fifteen positive isolates were further examined for their extent of alkaline protease production. The most potent producer was identified as Bacillus sp. Maximum enzyme activity was achieved in the presence of glucose (1% w/v) and ammonium chloride (1% w/v) at pH 10.5 and 40 degrees C over a 20 h incubation. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:201 / 203
页数:3
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