Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus

被引：42
|
作者：
Singh, J [1 ]
Vohra, RM [1 ]
Sahoo, DK [1 ]
机构：
[1] Inst Microbial Technol, Biochem Engn Res & Proc Dev Ctr, Chandigarh 160036, India
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 10期
关键词：
alkaline protease; Bacillus sphaericus; detergent compatibility; obligate alkalophilic; silver recovery;
D O I：
10.1023/A:1005502824637
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An obligate alkalophilic Bacillus sphaericus strain, isolated from alkaline soils in the Himalaya, produced an extracellular protease which was optimally active at 50-55 degrees C and pH 10.5. The enzyme was stable in presence of 500 mg chlorine l(-1) and as a detergent additive. Its stability in presence of laundry detergents was comparable to that of commercial proteases. The gelatin layer in 25 g of used X-ray films was efficiently hydrolyzed within 12 min at 50 degrees C, pH 11.0 and 25 U protease/ml.
引用
收藏
页码：921 / 924
页数：4
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