Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis

被引：0
|
作者：
Fukusaki, E
Oishi, T
Tanaka, H
Kajiyama, S
Kobayashi, A
机构：
[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan
[2] Nitto Denko Corp, Med Prod Sector, Ibaraki, Osaka 5678680, Japan
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 2001年 / 56卷 / 9-10期
关键词：
taxol; HeLa cells; DNA microarray;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.
引用
收藏
页码：814 / 819
页数：6
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