Conformation-based assay of tau protein aggregation

被引:10
|
作者
Fichou, Yann [1 ]
Eschmann, Neil A. [1 ]
Keller, Timothy J. [1 ]
Han, Songi [1 ]
机构
[1] Univ Calif Santa Barbara, Santa Barbara, CA 93106 USA
来源
关键词
INTRINSICALLY DISORDERED PROTEINS; PAIR DISTANCE DISTRIBUTIONS; ELECTRON DOUBLE-RESONANCE; IMPROVED SENSITIVITY; HELICAL FILAMENTS; BAND INVERSION; DEER; TAUOPATHY; FIBRILS; BRAINS;
D O I
10.1016/bs.mcb.2017.06.008
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Amyloid fiber-forming proteins are predominantly intrinsically disordered proteins (IDPs). The protein tau, present mostly in neurons, is no exception. There is a significant interest in the study of tau protein aggregation mechanisms, given the direct correlation between the deposit of b-sheet structured neurofibrillary tangles made of tau and pathology in several neurodegenerative diseases, including Alzheimer's disease. Among the core unresolved questions is the nature of the initial step triggering aggregation, with increasing attention placed on the question whether a conformational change of the IDPs plays a key role in the early stages of aggregation. Specifically, there is growing evidence that a shift in the conformation ensemble of tau is involved in its aggregation pathway, and might even dictate structural and pathological properties of mature fibers. Yet, because IDPs lack a well-defined 3D structure and continuously exchange between different conformers, it has been technically challenging to characterize their structural changes on-pathway to aggregation. Here, we make a case that double spin labeling of the b-sheet stacking region of tau combined with pulsed double electron-electron resonance spectroscopy is a powerful method to assay conformational changes occurring during the course of tau aggregation, by probing intramolecular distances around aggregation-prone domains. We specifically demonstrate the potential of this approach by presenting recent results on conformation rearrangement of the beta-sheet stacking segment VQIINK (known as PHF6*) of tau. We highlight a canonical shift of the conformation ensemble, on-pathway and occurring at the earliest stage of aggregation, toward an opening of PHF6*. We expect this method to be applicable to other critical segments of tau and other IDPs.
引用
收藏
页码:89 / 112
页数:24
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