In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy

被引：0
|
作者：
Fukushima, Shu-ichiro [1 ]
Tanaka, Yuji [1 ]
Hase, Eiji [2 ,3 ]
Takeichi, Kazuma [2 ]
Yasui, Takeshi [1 ,2 ]
机构：
[1] Osaka Univ, Grad Sch Engn Sci, 1-3 Machikaneyama, Toyonaka, Osaka 5608531, Japan
[2] Tokushima Univ, Grad Sch Technol Ind & Social Sci, 2-1 Minami Josanjima, Tokushima, Tokushima 7708506, Japan
[3] JASRI SPring 8, 1-1-1 Sayo, Sayo, Hyogo 6795198, Japan
来源：
BIOMEDICAL IMAGING AND SENSING CONFERENCE | 2018年 / 10711卷
关键词：
second-harmonic-generation; third-harmonic-generation; collagen; wound; healing; skin; GENERATION MICROSCOPY; BIOLOGY;
D O I：
10.1117/12.2319458
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Precise assessment of wound healing degree is important to avoid infection and achieve good prognosis without remaining of keloid or hypertrophic scars. In this paper, we apply the combination of second-harmonic-generation (SHG) microscopy with third-harmonic-generation (THG) microscopy for incised wound animal model, and visualize collagen dynamics during the wound healing process in time series in situ. Due to the difference of contrast mechanism, SHG image visualizes distribution of dermal collagen fibers whereas THG image visualizes surface of the epidermis. SHG/THG microscopy enable us to track the phase of wound healing for the same subject from the viewpoint of the temporal and spatial dynamic of collagen fibers and the epidermis surface.
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页数：4
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