Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse

被引：11

作者：

Chiers, Koen ^{[1
]}

Deschaght, Pieter ^{[2
]}

De Baere, Thierry ^{[2
,3
]}

Dabrowski, Slawomir ^{[4
]}

Kotlowski, Roman ^{[4
]}

De Clercq, Dominique ^{[5
]}

Ducatelle, Richard ^{[1
]}

Vaneechoutte, Mario ^{[2
]}

机构：

[1] Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Poultry Dis, B-9820 Merelbeke, Belgium

[2] Univ Ghent, Fac Med, Dept Clin Chem Microbiol & Immunol, Lab Bacteriol Res, B-9000 Ghent, Belgium

[3] Natl Inst Criminalistiek & Criminologie, B-1120 Brussels, Belgium

[4] Gdansk Univ Technol, Dept Microbiol, Gdansk, Poland

[5] Univ Ghent, Fac Vet Med, Dept Large Anim Internal Med, B-9820 Merelbeke, Belgium

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2012年 / 35卷 / 04期

关键词：

Mycobacterium avium subspecies silvaticum; Horse; Tuberculosis; RESTRICTION ANALYSIS; SP-NOV; TUBERCULOSIS; COMPLEX; INFECTION; PARATUBERCULOSIS; DIFFERENTIATION; PROPOSAL; ANIMALS;

D O I：

10.1016/j.cimid.2012.01.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Routine cultivation methods are able to distinguish between isolates of the Mycobacterium avium and the Mycobacterium tuberculosis complex. However, molecular tools are needed to further identify the several subspecies in the M. avium complex, especially for the subspecies avium and silvaticum. A rapid technique using Hhal restriction digestion of a 349 bp amplification product of the 85B antigen (alpha-antigen) gene was used for the identification of M. avium subsp. silvaticum in a three-year-old gelding presenting with caseous, necrotizing, granulomatous lesions. The result was confirmed by sequencing of the 85B antigen gene. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：303 / 307

页数：5

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