Insulin and intracellular calcium responsiveness to glucagon-like peptide-1 and pituitary adenylate cyclase-activating peptide by dispersed adult porcine islet cells

被引:9
|
作者
Davalli, AM
Bertuzzi, F
Meoni, C
Scaglia, L
Socci, C
Pozza, G
Pontiroli, AE
机构
[1] San Raffaele Sci Inst, Unit Metab Dis, I-20132 Milan, Italy
[2] San Raffaele Sci Inst, Unit Expt Surg, I-20132 Milan, Italy
[3] San Raffaele Sci Inst, Dept Med, I-20132 Milan, Italy
关键词
D O I
10.1097/00007890-199901150-00028
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for beta-cell replacement therapy in humans. Methods. We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++](i)) by adult porcine islet cells. Results, Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++](i), whereas the addition of GLP-1 and PACAP induced [Ca++](i) increases in 86% and 93% of cells, respectively. Conclusions. The good insulin and [Ca++](i) responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
引用
收藏
页码:174 / 176
页数:3
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