High-speed 3D imaging based on structured illumination and electrically tunable lens

被引：7
|
作者：
Wang, Dongping [1 ,2 ]
Meng, Yunlong [1 ,2 ]
Chen, Dihan [1 ,2 ]
Yam, Yeung [1 ]
Chen, Shih-Chi [1 ,2 ]
机构：
[1] Chinese Univ Hong Kong, Dept Mech & Automat Engn, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
来源：
CHINESE OPTICS LETTERS | 2017年 / 15卷 / 09期
基金：
中国国家自然科学基金;
关键词：
MICROSCOPY; LIVE; CELLS;
D O I：
10.3788/COL201715.090004
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens (ETL), where the ETL performs fast axial scanning at hundreds of Hz. In the system, a digital micro-mirror device (DMD) is utilized to rapidly generate structured images at the focal plane in synchronization with the axial scanning unit. The scanning characteristics of the ETL are investigated theoretically and experimentally. Imaging experiments on pollen samples are performed to verify the optical cross-sectioning and fast axial scanning capabilities. The results show that our system can perform fast axial scanning and three-dimensional (3D) imaging when paired with a high-speed camera, presenting an economic solution for advanced biological imaging applications.
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页数：4
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