In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

被引：18
|
作者：
Bantawa, P. [1 ]
Saha-Roy, O. [1 ]
Ghosh, S. Kumar [1 ]
Mondal, T. Kumar [1 ]
机构：
[1] Uttar Banga Krishi Viswavidalaya, Fac Hort, Biotechnol Lab, Cooch Behar 736165, W Bengal, India
来源：
BIOLOGIA PLANTARUM | 2011年 / 55卷 / 01期
关键词：
auxins; callus induction; cytokinins; picroside; ROYLE EX BENTH; CLONAL PROPAGATION; CULTURE; KURROA; HERB;
D O I：
10.1007/s10535-011-0024-8
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A reproducible in vitro regeneration system for Nepalese kutki (Picrorhiza scrophulariiflora Pennell) was developed from in vitro leaf derived callus. Induction of more than seven shoot buds per explant was achieved on Woody plant medium (WPM) supplemented with 0.53 mu M alpha-napthaleneacetic acid (NAA) and 0.23 mu M kinetin (KIN). The shoots were elongated on WPM supplemented with 0.44 mu M 6-benzylaminopurine (BAP) and rooted on WPM supplemented with 5.3 mu M NAA within 2 weeks. The random amplified polymorphic DNA (RAPD) analysis indicated genetic uniformity of the micropropagated plants with its donor plants.
引用
收藏
页码：169 / 172
页数：4
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