DETERMINATION OF HYDROCORTISONE IN PHARMACEUTICAL DRUG BY TLC WITH DENSITOMETRIC DETECTION IN UV

被引：12

作者：

Pyka, Alina ^{[1
]}

Babuska-Roczniak, Magdalena ^{[1
]}

Bochenska, Paulina ^{[1
]}

机构：

[1] Med Univ Silesia, Dept Analyt Chem, Fac Pharm, PL-41200 Sosnowiec, Poland

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2011年 / 34卷 / 09期

关键词：

densitometry; hydrocortisone; pharmaceutical analysis; quantitative analysis; spectrodensitometry; spectrophotometry; TLC; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIN-LAYER-CHROMATOGRAPHY; ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; ISONICOTINIC-ACID HYDRAZIDE; FLUORESCENCE DERIVATIZATION; MASS-SPECTROMETRY; HUMAN-PLASMA; CORTISOL; CORTICOSTEROIDS; URINE;

D O I：

10.1080/10826076.2011.563891

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adsorption thin layer chromatography with densitometry has been established for the identification and the quantification of hydrocortisone in tablets. UV densitometry was performed in absorbance mode at 250nm. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. The TLC-densitometric method was also compared with a pharmacopeil UV-spectrophotometric method for the assay of hydrocortisone, and the results confirmed statistically that the TLC-densitometric method can be used as a substitute method.

引用

页码：753 / 769

页数：17

共 50 条

[31] Development and Validation of TLC-Densitometric Method for the Quantification of a Steroidal Drug, Danazol in its Pharmaceutical Formulations
Musharraf, Syed Ghulam
ul Arfeen, Qamar
Shoaib, Muhammad
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2012, 25 (04) : 331 - 337
[32] TLC-densitometric analysis of alpha-escin in bulk drug substance and in pharmaceutical dosage forms
Dolowy, Malgorzata
Pyka-Pajak, Alina
Filip, Katarzyna
Zagrodzka, Joanna
CURRENT ISSUES IN PHARMACY AND MEDICAL SCIENCES, 2015, 28 (03) : 186 - 191
[33] Chemometric Approach to Selectivity in TLC with Densitometric Detection
Komsta, Lukasz
Kobylka, Mariola
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2013, 51 (05) : 400 - 405
[34] TLC-Densitometric Determination of Tenoxicam and Its Degradation Products in Pharmaceutical Preparations and after Hydrolysis in Solutions
Starek, Malgorzata
Krzek, Jan
Tarsa, Monika
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2011, 24 (04) : 337 - 343
[35] TLC-DENSITOMETRIC DETERMINATION OF TOLPERISONE AND ITS IMPURITIES 4-METHYLPROPIOPHENONE AND PIPERIDINE IN PHARMACEUTICAL PREPARATIONS
Hubicka, Urszula
Krzek, Jan
Zuromska-Witek, Barbara
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2012, 35 (10) : 1325 - 1335
[36] TLC-Densitometric Determination of Tenoxicam and Its Degradation Products in Pharmaceutical Preparations and after Hydrolysis in Solutions
Małgorzata Starek
Jan Krzek
Monika Tarsa
JPC – Journal of Planar Chromatography – Modern TLC, 2011, 24 : 337 - 343
[37] Stability-indicating TLC-Densitometric Determination of Nebivolol Hydrochloride in Bulk and Pharmaceutical Dosage Form
Shirkhedkar, Atul A.
Bugdane, Prasad M.
Surana, Sanjay J.
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2010, 48 (02) : 109 - 113
[38] Determination of Oxazepam in Pharmaceutical Formulation by HPTLC UV-Densitometric and UV-Derivative Spectrophotometry Methods
Koba, Marcin
Koba, Katarzyna
Baczek, Tomasz
ANALYTICAL LETTERS, 2009, 42 (12) : 1831 - 1843
[39] Stability-indicating UPLC, TLC-densitometric and UV-spectrophotometric methods for alcaftadine determination
Razeq, Sawsan A. Abdel
Aziz, Shimaa E. Abdel
Ahmed, Nermin S.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2021, 1177
[40] UV-DENSITOMETRIC DETERMINATION OF SPARFLOXACIN AND ITS APPLICATION TO THE ASSAY IN PHARMACEUTICAL DOSAGE FORMS
Sharma, M. C.
Sharma, Smita
JOURNAL OF OPTOELECTRONIC AND BIOMEDICAL MATERIALS, 2010, 2 (04): : 191 - 195

← 1 2 3 4 5 →