DETERMINATION OF HYDROCORTISONE IN PHARMACEUTICAL DRUG BY TLC WITH DENSITOMETRIC DETECTION IN UV

被引：12
|
作者：
Pyka, Alina [1 ]
Babuska-Roczniak, Magdalena [1 ]
Bochenska, Paulina [1 ]
机构：
[1] Med Univ Silesia, Dept Analyt Chem, Fac Pharm, PL-41200 Sosnowiec, Poland
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2011年 / 34卷 / 09期
关键词：
densitometry; hydrocortisone; pharmaceutical analysis; quantitative analysis; spectrodensitometry; spectrophotometry; TLC; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIN-LAYER-CHROMATOGRAPHY; ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; ISONICOTINIC-ACID HYDRAZIDE; FLUORESCENCE DERIVATIZATION; MASS-SPECTROMETRY; HUMAN-PLASMA; CORTISOL; CORTICOSTEROIDS; URINE;
D O I：
10.1080/10826076.2011.563891
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Adsorption thin layer chromatography with densitometry has been established for the identification and the quantification of hydrocortisone in tablets. UV densitometry was performed in absorbance mode at 250nm. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. The TLC-densitometric method was also compared with a pharmacopeil UV-spectrophotometric method for the assay of hydrocortisone, and the results confirmed statistically that the TLC-densitometric method can be used as a substitute method.
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页码：753 / 769
页数：17
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