Genetic diversity in cowpea (Vigna unguiculata) using isozyme electrophoresis

被引：5
|
作者：
Reis, CM [1 ]
Frederico, AM [1 ]
机构：
[1] Escola Super Agraria Castelo Brnco, Lab Biol Vegetal, P-6000 Castelo Branco, Portugal
来源：
PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON MOLECULAR MARKERS FOR CHARACTERIZING GENOTYPES AND IDENTIFYING CULTIVARS IN HORTICULTURE | 2001年 / 546期
关键词：
cowpea; Vigna unguiculata; cultivar groups; polymorphic loci; esterase;
D O I：
10.17660/ActaHortic.2001.546.68
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Accessions of cultivated cowpea (V. unguiculata ssp. unguiculata cv. gr. unguiculata, cv. gr. sesquipedalis and cv. gr, biflora) from different countries were evaluated for isozyme variability. Zymograms were obtained by polyacrylamide gel electrophoresis using the PhastSystem (TM) and eight enzyme systems were studied. Cowpea accessions studied were characterised by very low genetic diversity. Accessions were monomorphic for almost all loci tested except for the esterase enzyme system. Comparative analysis of esterase zymograms made it possible to identify some of the accessions studied. However, the cultivated groups biflora and sesquipedalis could not be distinguished from one another or from the cv. gr. unguiculata.
引用
收藏
页码：497 / 501
页数：5
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