A ribonuclease from sclerotia of the edible mushroom Pleurotus tuber-regium

被引：23

作者：

Wang, HX ^{[1
]}

Ng, TB

Ooi, VEC

机构：

[1] Chinese Univ Hong Kong, Dept Biol, Shatin, NT, Peoples R China

[2] Chinese Univ Hong Kong, Dept Biochem, Shatin, NT, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 250卷 / 03期

关键词：

ribonuclease; mushroom; Pleurotus; isolation;

D O I：

10.1006/bbrc.1998.9361

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A ribonuclease with a molecular weight of 29 kDa as determined by FPLC-gel filtration on Superose 12 was isolated from the sclerotia of the mushroom Pleurotus tuber-regium using a procedure involving extraction with aqueous buffer, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affigel blue gel, ion exchange chromatography on CM-cellulose, and FPLC on Mono S, The protein was unadsorbed on DEAE-cellulose but adsorbed on Affigel blue gel and CM-cellulose, It was homodimeric, made up of two identical subunits, each with a molecular weight of 14.5 kDa as witnessed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It exhibited potent ribonucleolytic activity toward Poly G. Its ribonucleolytic activity was resistant to heating at 100 degrees C for 30 min, but was inhibited by HgCl2, ZnSO4, NiSO4, CaCl2, and Pb(NO3)(2). (C) 1998 Academic Press.

引用

页码：544 / 546

页数：3

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