Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties

被引：10

作者：

Cui, Jin ^{[1
,2
,3
]}

Lethu, Sebastien ^{[1
,2
,3
]}

Yasuda, Tomokazu ^{[1
]}

Matsuoka, Shigeru ^{[1
,2
,3
]}

Matsumori, Nobuaki ^{[1
]}

Sato, Fuminori ^{[2
,3
]}

Murata, Michio ^{[1
,2
,3
]}

机构：

[1] Osaka Univ, Dept Chem, Toyonaka, Osaka 5600043, Japan

[2] Osaka Univ, Lipid Active Struct Project, ERATO, JST, Toyonaka, Osaka 5600043, Japan

[3] Osaka Univ, Project Res Ctr Fundamental Sci, Toyonaka, Osaka 5600043, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2015年 / 25卷 / 02期

关键词：

Lipid raft; Phase separation; Phosphatidylcholine; Solid-state H-2 NMR; LIPID RAFTS; PHASE-DIAGRAM; DIHYDROSPHINGOMYELIN; SPHINGOMYELIN; HIV-1; HYDRATION; BILAYERS;

D O I：

10.1016/j.bmcl.2014.11.072

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-H-2(2)] oleic acid, and newly synthesized [C6-H-2(2)] DOPC. In raft-like oriented bilayers, [C6-H-2(2)] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：203 / 206

页数：4

共 46 条

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