Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA

被引：27

作者：

Wong, Nicholas C. ^{[1
,7
]}

Ng, Jane ^{[1
]}

Hall, Nathan E. ^{[2
,3
]}

Lunke, Sebastian ^{[4
]}

Salmanidis, Marika ^{[5
]}

Brumatti, Gabriela ^{[5
]}

Ekert, Paul G. ^{[5
]}

Craig, Jeffrey M. ^{[6
]}

Saffery, Richard ^{[1
]}

机构：

[1] Univ Melbourne, Canc & Dis Epigenet Grp, Murdoch Childrens Res Inst, Dept Paediat,Royal Childrens Hosp, Parkville, Vic 3052, Australia

[2] La Trobe Univ, Dept Genet, Melbourne, Vic, Australia

[3] Victorian Life Sci Computat Initiat, Life Sci Computat Ctr, Melbourne, Vic 3010, Australia

[4] Univ Melbourne, Translat Genom Lab, Ctr Translat Pathol, Dept Pathol, Parkville, Vic 3010, Australia

[5] Walter & Eliza Hall Inst Med Res, Cell Signalling & Cell Death Div, Parkville, Vic 3052, Australia

[6] Univ Melbourne, Murdoch Childrens Res Inst, Dept Paediat, Royal Childrens Hosp, Parkville, Vic 3052, Australia

[7] Austin Hosp, Ludwig Inst Canc Res, Olivia Newton John Canc & Wellness Ctr, Heidelberg, Vic 3084, Australia

来源：

GENOMICS | 2013年 / 102卷 / 01期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

DNA methylation; Infinium Bead Chip; Xeno-array; Human; Mouse; Epigenetics; GENE-EXPRESSION; ALIGNMENT; TOOL;

D O I：

10.1016/j.ygeno.2013.04.014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Illumina infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference mouse genome (mm9). We demonstrate reproducible measurements of DNA methylation at these probes in a range of mouse tissue samples and in a murine cell line model of acute myeloid leukaemia. In the absence of a mouse counterpart, the Infinium Human Methylation BeadChip arrays have utility for methylation profiling in non-human species. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：38 / 46

页数：9

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