Serial section Raman tomography with 10 times higher depth resolution than confocal Raman microscopy

被引：6

作者：

Boehm, Thomas ^{[1
,2
]}

Moroni, Riko ^{[3
]}

Thiele, Simon ^{[1
,2
]}

机构：

[1] Forschungszentrum Julich, Helmholtz Inst Erlangen Nurnberg Renewable Energy, D-91058 Erlangen, Germany

[2] Friedrich Alexander Univ Erlangen Nurnberg, Dept Chem & Biol Engn, Erlangen, Germany

[3] Univ Freiburg, IMTEK Dept Microsyst Engn, Lab MEMS Applicat, Freiburg, Germany

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2020年 / 51卷 / 07期

关键词：

polymer; Raman imaging; serial sectioning; tomography; 3D reconstruction;

D O I：

10.1002/jrs.5878

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Confocal Raman microscopy enables 3D imaging of various samples solely based on chemical contrast. However, optical artifacts impair resolution and image quality in subsurface imaging. With serial section Raman tomography, we show that serial ultrathin and semithin sectioning by ultramicrotomy can successfully be combined with subsequent confocal Raman imaging. This new 3D Raman imaging technique reaches a depth resolution of up to 100 nm, which is about 10-fold better than in confocal Raman microscopy. Structurally complex and optically inhomogeneous samples can not only be imaged, but also be used to quantify structural parameters. Serial section Raman tomography is a promising method for materials science and possibly also for life sciences.

引用

页码：1160 / 1171

页数：12

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