Determination of bovine blood oleandrin by high-performance liquid chromatography and postcolumn derivatization

被引：8
|
作者：
Hamada, K
Iwamoto, A
Miyazaki, S
Yamanaka, N
Guruge, KS
机构：
[1] Natl Inst Anim Hlth, Toxico Biochem Sect, Tsukuba, Ibaraki 3050856, Japan
[2] Kumamoto Cent Anim Hyg Serv Ctr, Shimonoseki, Yamaguchi 8614215, Japan
[3] Himeji Anim Hyg Serv Ctr, Himeji, Hyogo 6700081, Japan
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2002年 / 40卷 / 09期
关键词：
D O I：
10.1093/chromsci/40.9.555
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic procedure with a postcolumn fluorescence derivatization is developed for the analysis of oleandrin in bovine blood. Oleandrin is separated by an octadecylsilane-bonded column with a mobile phase containing dehydroascorbic acid. The effluent of the column is mixed with concentrated hydrochloric acid and passed through poly(tetrafluoroethylene) tubing maintained at 70°C. The resultant fluorophores are detected at 465 nm with excitation at 348 nm. Simple solid-phase extraction using Sep-Pak tC2 is effective for sample purification. We found the minimal detectable quantity of oleandrin in plasma to be 1.5 ng/mL at a signal-to-noise ratio of 3:1.
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页码：515 / 518
页数：4
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