Screening and Selection of Aspergillus flavus Strain for Alkaline Protease Production by Submerged Fermentation

被引：0

作者：

Rani, M. Roja ^{[1
]}

Prasad, N. N. ^{[2
]}

机构：

[1] JNTUK, AIET, Dept Biotechnolgy, Satuluru 522006, Nrt, India

[2] Vignan Sch PG Studies, Guntur, Andhra Pradesh, India

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2012年 / 6卷 / 03期

关键词：

Alkaline protease; Isolation; Screening; Aspergillus flavus; SOLID-SUBSTRATE FERMENTATION; PROTEINASE; PURIFICATION; FUMIGATUS;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

20 fungal strains were screened from alkaline soils such as black cotton soil, groundnut field, milk processing unit, Kotappakonda hill area in Guntur Dist and Tirumala in Chittur District, Andhrapradesh, India. KS2 exhibited the prominent zone of clearance on modified seed medium at pH 9.0. The isolated species were identified basing on colony morphology,microscopic morphology,growth presence on temperature and pH of test fungal organism were identified as Aspergillus flavus. The alkalophillic isolate Aspergillus flavus shows maximum alkaline protease enzyme activity was (2000 U/mL) at pH 8.5, 40 degrees C for 72 hr. These characteristics its potential use in alkaline protease production.

引用

页码：1265 / 1271

页数：7

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