Bioaccumulation and persistence of faecal bacterial and viral indicators in Mytilus edulis and Crassostrea gigas

被引:17
|
作者
Olalemi, A. [1 ,3 ]
Baker-Austin, C. [2 ]
Ebdon, J. [1 ]
Taylor, H. [1 ]
机构
[1] Univ Brighton, Sch Environm & Technol, Environm & Publ Hlth Res Grp, Brighton, E Sussex, England
[2] Ctr Environm Fisheries & Aquaculture Sci, Weymouth, Dorset, England
[3] Fed Univ Technol Akure, Dept Microbiol, PMB 704, Akure, Ondo State, Nigeria
关键词
Exposure; Monitoring; Human health; Microbial source tracking; Shellfish; Surrogates; Viruses; PERNA-PERNA LINNAEUS; BIVALVE SHELLFISH; SOURCE TRACKING; MUSSEL; PATHOGENS; VIRGINICA; ENTEROCOCCUS; ELIMINATION; TEMPERATURE; DEPURATION;
D O I
10.1016/j.ijheh.2016.06.002
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
This study investigated the response of two shellfish species - mussels (Mytilus edulis) and oysters (Crassostrea gigas) to microbial contamination in order to gain a better understanding of the bioaccumulation and persistence of microorganisms under controlled conditions. M. edulis and C gigas were placed in sterile laboratory-prepared artificial seawater and initial tests were carried out to ensure both faecal indicator bacteria (FIB) and bacteriophages were below detection limits. FIB and phages were isolated, purified and dosed into experimental tanks containing the shellfish species. The GB124 phages were bioaccumulated to the highest concentration in M. edulis (1.88 Log(10)) and C. gigas (1.27 Log(10)) after 24 h. Somatic coliphages were bioaccumulated to the highest concentration in both M. edulis (4.84 log(10)) and C gigas (1.73 log(10)) after 48 h. F-RNA phages were bioaccumulated to the highest concentration in M. edulis (3.51 log(10)) after 6 h but were below detection limit in C. gigas throughout the exposure period. Escherichia coli, faecal coliforms and intestinal enterococci were bioaccumulated to the highest concentrations in M. edulis (5.05 log(10), 5.06 log(10) and 3.98 log(10) respectively) after 48 h. In C. gigas, E. coli reached a maximum concentration (5.47 log(10)) after 6 h, faecal coliforms (5.19 log(10)) after 12 h and intestinal enterococci (3.23 log(10)) after 24 h. M. edulis bioaccumulated phages to a greater extent than the faecal bacteria, and in both shellfish species, faecal bacteria persisted for longer periods over 48 h than the phages. This study highlights significant variation in the levels and rate of accumulation and persistence with respect to both shellfish species and the indicators used to assess risk. The results suggest that phage-based indicators could help elucidate risks to human health associated with pathogenic organisms. Crown Copyright (C) 2016 Published by Elsevier GmbH. All rights reserved.
引用
收藏
页码:592 / 598
页数:7
相关论文
共 49 条
  • [31] Feeding current characteristics of three morphologically different bivalve suspension feeders, Crassostrea gigas, Mytilus edulis and Cerastoderma edule, in relation to food competition
    Troost, Karin
    Stamhuis, Eize J.
    van Duren, Luca A.
    Wolff, Wim J.
    MARINE BIOLOGY, 2009, 156 (03) : 355 - 372
  • [32] Relative impacts of the invasive Pacific oyster, Crassostrea gigas, over the native blue mussel, Mytilus edulis, are mediated by flow velocity and food concentration
    Joyce, Patrick W. S.
    Kregting, Louise T.
    Dick, Jaimie T. A.
    NEOBIOTA, 2019, (45) : 19 - 37
  • [33] Field clearance of an intertidal bivalve bed: relative significance of the co-occurring blue mussel Mytilus edulis and Pacific oyster Crassostrea gigas
    Vismann, Bent
    Holm, Mark Wejlemann
    Davids, Jens Kristian
    Dolmer, Per
    Pedersen, Morten Foldager
    Blanda, Elisa
    Christensen, Helle Torp
    Nielsen, Pernille
    Hansen, Benni Winding
    AQUATIC BIOLOGY, 2016, 25 : 107 - 119
  • [34] VIRAL AND BACTERIAL-CONTAMINATION OF MUSSELS (MYTILUS-EDULIS) EXPOSED IN AN UNPOLLUTED MARINE-ENVIRONMENT
    JEHLPIETRI, C
    HUGUES, B
    DELOINCE, R
    LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (03) : 126 - 129
  • [35] SILVER AND LEAD CONTAMINATION OF THE OYSTER CRASSOSTREA-GIGAS AND THE MUSSEL MYTILUS-EDULIS IN FRENCH COASTAL WATERS - A SECONDARY ION EMISSION MICROANALYTICAL STUDY
    CHASSARDBOUCHAUD, C
    GALLE, P
    ESCAIG, F
    COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1985, 300 (01): : 3 - 8
  • [36] Coexistence of the native mussel, Mytilus edulis, and the invasive Pacific oyster, Crassostrea (Magallana) gigas, does not affect their growth or mortality, but reduces condition of both species
    Patrick W. S. Joyce
    David M. Smyth
    Jaimie T. A. Dick
    Louise T. Kregting
    Hydrobiologia, 2021, 848 : 1859 - 1871
  • [37] Coexistence of the native mussel, Mytilus edulis, and the invasive Pacific oyster, Crassostrea (Magallana) gigas, does not affect their growth or mortality, but reduces condition of both species
    Joyce, Patrick W. S.
    Smyth, David M.
    Dick, Jaimie T. A.
    Kregting, Louise T.
    HYDROBIOLOGIA, 2021, 848 (08) : 1859 - 1871
  • [39] Trophic subsidies of Crassostrea gigas, Mytilus edulis and Crepidula fornicata in the Bay of Mont Saint Michel (France):: A δ13C and δ15N investigation
    Riera, Pascal
    ESTUARINE COASTAL AND SHELF SCIENCE, 2007, 72 (1-2) : 33 - 41
  • [40] ORGAN-CULTURE ACTION OF THE NERVOUS GANGLIA OF THE MUSSEL (MYTILUS-EDULIS-L) ON THE GERMINAL LINE OF THE OYSTER (CRASSOSTREA-GIGAS TH) (MOLLUSCA, LAMELLIBRANCHIA)
    LUBET, P
    HERVE, Y
    BULLETIN DE LA SOCIETE ZOOLOGIQUE DE FRANCE-EVOLUTION ET ZOOLOGIE, 1981, 106 (03): : 382 - 383