Instrumental resolution effects in neutron scattering studies of protein dynamics

被引：9

作者：

Nickels, Jonathan D. ^{[1
,2
]}

机构：

[1] Oak Ridge Natl Lab, Joint Inst Neutron Sci, Oak Ridge, TN 37831 USA

[2] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

来源：

CHEMICAL PHYSICS | 2013年 / 424卷

关键词：

Neutron scattering; GFP; Protein dynamics; Instrumental resolution; Data treatment; GREEN FLUORESCENT PROTEIN; MOLECULAR MOTIONS; HYDRATION; TRANSITION; SPECTROSCOPY; SOLVENT; GLASSES; SOFT;

D O I：

10.1016/j.chemphys.2012.11.021

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this study, the dynamics of Green Fluorescent protein (GFP) are analyzed using three neutron scattering spectrometers. We focus on the effect of instrumental energy resolution in the analysis of the elastic incoherent structure factor (EISF) and mean square displacement (MSD). This topic still remains a source of controversy. Our data clearly demonstrate the presence of the resolution effect in the dynamic transition for hydrated protein and the onset of translational motions in hydration water consistent with previous results from quasielastic neutron scattering. The 190 K onset of motions in hydration water observed at similar to 1 ns is also consistent with a T-g of hydration water below 190 K. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：7 / 11

页数：5

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