Crystallization and preliminary X-ray crystallographic analysis of recombinant -mannosidase from Aspergillus niger

-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal -linked mannoside in various oligomeric saccharide structures. -Mannosidase from Aspergillus niger was expressed in Pichia pastoris and purified to clear homogeneity. -Mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 angstrom resolution. The crystal belonged to space group P1, with unit-cell parameters a = 62.37, b = 69.73, c = 69.90 angstrom, = 108.20, = 101.51, = 103.20 degrees. The parameters derived from the data collection indicate the presence of one molecule in the asymmetric unit.