Crystallization and preliminary X-ray crystallographic analysis of recombinant -mannosidase from Aspergillus niger

被引：0
|
作者：
Demo, Gabriel [1 ,2 ]
Fliedrova, Barbora [3 ,4 ]
Weignerova, Lenka [3 ]
Wimmerova, Michaela [1 ,2 ,5 ]
机构：
[1] Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic
[2] Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, Brno 62500, Czech Republic
[3] Acad Sci Czech Republic, Inst Microbiol, Lab Biotransformat, CR-14220 Prague, Czech Republic
[4] Charles Univ Prague, Dept Biochem, Fac Sci, Prague 12840, Czech Republic
[5] Masaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷
关键词：
-mannosidase; Aspergillus niger; BETA-MANNOSIDASE;
D O I：
10.1107/S1744309113002522
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal -linked mannoside in various oligomeric saccharide structures. -Mannosidase from Aspergillus niger was expressed in Pichia pastoris and purified to clear homogeneity. -Mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 angstrom resolution. The crystal belonged to space group P1, with unit-cell parameters a = 62.37, b = 69.73, c = 69.90 angstrom, = 108.20, = 101.51, = 103.20 degrees. The parameters derived from the data collection indicate the presence of one molecule in the asymmetric unit.
引用
收藏
页码：288 / 291
页数：4
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