Large-scale production of VHH antibody fragments by Saccharomyces cerevisiae

被引：41
|
作者：
Thomassen, YE
Meijer, W
Sierkstra, L
Verrips, CT
机构：
[1] Univ Utrecht, Dept Mol Cell Biol, NL-3584 CH Utrecht, Netherlands
[2] Univ Utrecht, Inst Biomembranes, NL-3584 CH Utrecht, Netherlands
[3] Unilever Res Labs Vlaardingen, NL-3133 AT Vlaardingen, Netherlands
[4] Biotechnol Applicat Ctr, Naarden, Netherlands
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 03期
关键词：
antibody; V-HH; bulk production; secretion; S; cerevisiae;
D O I：
10.1016/S0141-0229(01)00497-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We report the large-scale production of V-HH antibody fragments, raised against the azo-dye RR-6, by the yeast Saccharomyces cerevisiae. The secretion efficiency of the V-HH fragments was correlated with their overall hydrophobicity. With a 15 m(3) fed-batch fermentation, 1.3 kg V(HH)s were produced. Purification was done by removal of biomass and product concentration. During induction, the specific production rate q(p) was 0.213 g.[kg dryweight](-1).h(-1). (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：273 / 278
页数：6
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