Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR

被引：0
|
作者：
Romero, C [1 ]
Lopez-Goñi, I [1 ]
机构：
[1] Univ Navarra, Dept Microbiol, Pamplona 31008, Spain
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1999年 / 65卷 / 08期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.
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页码：3735 / 3737
页数：3
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