A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

被引：37

作者：

Yang, Kisuk ^{[1
]}

Han, Sewoon ^{[2
]}

Shin, Yoojin ^{[2
]}

Ko, Eunkyung ^{[1
]}

Kim, Jin ^{[1
]}

Park, Kook In ^{[3
,4
]}

Chung, Seok ^{[2
]}

Cho, Seung-Woo ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[2] Korea Univ, Sch Mech Engn, Seoul 136713, South Korea

[3] Yonsei Univ, Coll Med, Dept Pediat, Seoul 120752, South Korea

[4] Yonsei Univ, Coll Med, Project Med Sci BK21, Seoul 120752, South Korea

来源：

BIOMATERIALS | 2013年 / 34卷 / 28期

基金：

新加坡国家研究基金会;

关键词：

Microfluidic array; Neural stem cells; Hypoxia; Self-renewal; Differentiation; HUMAN NEURODEGENERATIVE DISORDERS; EXTRACELLULAR-MATRIX; DOPAMINERGIC DIFFERENTIATION; VASCULAR NICHE; PROLIFERATION; OXYGEN; CULTURE; HYDROXYLATION; NEUROGENESIS; PLURIPOTENCY;

D O I：

10.1016/j.biomaterials.2013.05.067

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：6607 / 6614

页数：8

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