A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

被引：37
|
作者：
Yang, Kisuk [1 ]
Han, Sewoon [2 ]
Shin, Yoojin [2 ]
Ko, Eunkyung [1 ]
Kim, Jin [1 ]
Park, Kook In [3 ,4 ]
Chung, Seok [2 ]
Cho, Seung-Woo [1 ]
机构：
[1] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea
[2] Korea Univ, Sch Mech Engn, Seoul 136713, South Korea
[3] Yonsei Univ, Coll Med, Dept Pediat, Seoul 120752, South Korea
[4] Yonsei Univ, Coll Med, Project Med Sci BK21, Seoul 120752, South Korea
来源：
BIOMATERIALS | 2013年 / 34卷 / 28期
基金：
新加坡国家研究基金会;
关键词：
Microfluidic array; Neural stem cells; Hypoxia; Self-renewal; Differentiation; HUMAN NEURODEGENERATIVE DISORDERS; EXTRACELLULAR-MATRIX; DOPAMINERGIC DIFFERENTIATION; VASCULAR NICHE; PROLIFERATION; OXYGEN; CULTURE; HYDROXYLATION; NEUROGENESIS; PLURIPOTENCY;
D O I：
10.1016/j.biomaterials.2013.05.067
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6607 / 6614
页数：8
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