Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris

被引：24

作者：

Wang, Lei ^{[1
]}

Lai, Chun-e ^{[1
]}

Wu, Qifeng ^{[1
]}

Liu, Junliang ^{[1
]}

Zhou, Maojun ^{[1
]}

Ren, Zhenghua ^{[1
]}

Sun, Dandan ^{[1
]}

Chen, Shangwu ^{[1
]}

Xu, Anlong ^{[1
]}

机构：

[1] Sun Yat Sen Zhongshan Univ, Coll Life Sci, State Key Lab Biocontrol, Dept Biochem,Guangdong Key Lab Pharmaceut Funct G, Guangzhou 510275, Guangdong, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2008年 / 43卷 / 10期

关键词：

antimicrobial peptide; HKABF; recombinant expression; purification; Pichia pastoris; antimicrobial activity;

D O I：

10.1016/j.procbio.2008.06.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HKABF, Hippocampus kuda antibacterial factor, is a putative antimicrobial peptide identified from the cDNA library of the brooding pouch of H. kuda Bleeker. The putative mature peptide, fused with a 6His-tag, an anionic peptide (SAPLNPDNID) and to an enterokinase cleavage site was expressed. in Pichia pastoris. The mature HKABF peptide, released by enterokinase cleavage, showed antimicrobial activity against Staphylococcus aureus and Staphylococcus saprophyticus at low concentrations (0.5-2.5 mu g/ml). The recombinant mature peptide was heat-stable and had low hemolytic activity. Circular dichroism (CD) spectroscopy data indicates its classification as a CS alpha beta-type antimicrobial peptide. This study serves as an impetus for the production and further research of this antimicrobial peptide. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1124 / 1131

页数：8

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